COBRE: Smooth Muscle Plasticity Phase III

COBRE: Smooth Muscle Plasticity Phase III

Photos of students conducting research

The Flow Cytometry and Single Cell Molecular Expression Core Facilities utilize state-of-the-art technologies to offer high quality single cell analysis, genetic engineering, and single cell RNA expression analysis to all research faculty and students in the Nevada System of Higher Education (NSHE) at extremely competitive rates.

Core B

Core B

The Fluorescence-Activated Cell Sorting/Flow Cytometry Shared Resource Laboratory (FACS/FCMSRL) provides analytical fluorescence-based flow cytometry and cell sorting. The FACS/FCMSRL provides the following services:

  • assisting in designing, executing and analyzing flow cytometric experiments
  • titrating and testing labeled antibodies
  • providing laboratory and classroom training in flow cytometry

It serves investigators from laboratories in the University of Nevada School of Medicine, the University of Nevada Reno, and from other public and private laboratories in the area.

CORE C

Core C

The Single Cell Molecular Expression Core (SCME) provides investigators and other researchers in the Nevada System of Higher Education (NSHE) with the following services:

  • Automated single cell capture followed by cDNA preparation using the Fluidigm C1 Single Cell Auto-Prep system
  • High throughput quantitative PCR assays and targeted template enrichment using the Fluidigm BioMark HD system
  • Small noncoding RNA deep sequencing (sncRNA-Seq) or targeted DNA deep sequencing using the Ion Torrent PGM sequencer
  • Ribosome profiling to determine translational status of mRNAs in a given tissue/organ using RiboTag mice and RNA-Seq
  • Manipulation of a gene of interest to achieve over-expression, reduced expression, complete inactivation, or replacement with another gene using the latest genome editing technologies including ZFNs, TALENs and Cas9-CRISPR in cultured cells.
  • Assistance in the designing, execution and analysis of next-generation deep sequencing and genome editing experiments
Pilot Grant Program

Pilot Grant Program

Pilot Grants are awarded to investigators to utilize the Cores to develop new, innovative approaches to generate proof of principle data to accelerate successful extramural grant funding.